Research Kits

RESEARCH KITS

(Silica Column Based)

Plasmid DNA Purification (Spin Column Based)

For rapid and convenient plasmid DNA preparations

• High yield of plasmid DNA
• Purify plasmids up to 10 kb
• Plasmid DNA is ready for restriction digestion, bacterial transformation, sequencing and more
• Rapid spin-column format

Plasmid Mini Prep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 12,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more.

Taq DNA Polymerase With 10X Reaction Buffer

Highly reliable and reproducible reactions

• Recombinant source
• Preserved functionality at wide range of temperatures
• No detectable levels of contaminating endonucleases or exonucleases
• Taq DNA Polymerase with 10× reaction buffer with MgCl2
• Compatible with the use of modified nucleotides (dUTP, dITP, biotin-, digoxigenin- and fluorescently-labeled nucleotides)

Applications:

• Routine PCR
• Preparation of PCR products for cloning
• Primer extension
• Microarray analysis
• DNA labeling
• DNA sequencing
  and many more…

Taq Polymerase Assay Buffers, With/Without MgCl2

Standalone buffer compatible with Taq DNA Polymerase

Foreign activity :

DNase, RNase, none detected

Application:

For routine PCR amplifications

PCR Master Mix kit (2X)

A ready-to-use solution optimized for robust amplification of high yield products

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in convenient sizes.

MODIFICATION ENZYMES

Through continued research in these areas, we are committed to driving the innovations that allow us to offer maximum convenience and performance. The result is a consistently superior enzyme that delivers on its promised performance and activity.

DNA, PLASMID AND PHAGE VECTORS

Lambda DNA

Phage lambda DNA is a common substrate for restriction endonucleases and for generating DNA size marker fragments. Linear double-stranded lambda bacteriophage (cI857 Sam7) DNA, 48502 base pairs with a molecular weight of 31.5 × 106 Da. Isolated from a heat-inducible lysogenic E.coli W3110 strain.

Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by
recombination.

NUCLEIC ACID ELECTROPHORESIS CHEMICALS & REAGENTS

Lambda DNA/Eco RI Digest

• For sizing and approximate quantification of large DNA
  fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Agarose (Low EEO)

• High purity biotechnology grade agarose
• High purity, free from nucleases
• Exceptional DNA and RNA separation
• Long term storage at room temperature
• QC controlled for consistency and reliability

Agarose

ABBES BIOTECH offers ideal Biotechnology grade Agarose for routine analysis of nucleic acids by gel electrophoresis and blotting. Each gel sharply resolves DNA and provides consistent resolution. This Biotechnology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background of dye. Due to its low EEO, DNA will have a high electrophoretic mobility. The gels are easy to handle even at low agarose percentages and are recommended for low-percentage gels (0.7–2.0%) required for applications such as DNA fingerprinting, PCR or restriction digest analyses.

Intended Use:

This product use is only for Research purpose. This product is not for use in in vitro Diagnostic Procedures, drug use, or for administration to humans or animals.

Storage Conditions:

Agarose should be stored tightly sealed at room temperature.

Proteomics Products

30% Acrylamide/ Bis-acrylamide solution, (29:1)

Ready-to-use high-purity (99.9%) solution

Reduce inhalation and contact hazards associated with weighing and preparing acrylamide and bis-acrylamide solutions.

Precise composition and high-purity provide uniformity of gel matrices, consistent polymerization, and run-to-run reproducibility.

provides30% Acrylamide/Bis-acrylamide, 29:1 (3.3% crosslinker) solution as a faster and safer alternative to handling powdered acrylamide and bis-acrylamide.
These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bisacrylamide.
The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions.

Applications:

• DNA sequencing
• Protein separation

Tris-SDS Buffer, pH: 8.8 (Gel Casting Buffer – For PAGE)

Ready-to-use buffer for SDS-PAGE

• Reduce preparation time and eliminate a source of error
• No reagents to weigh or filter; just dilute with distilled deionized water.
• High purity buffer; free from contaminants
• Manufactured under strict quality controls for reliability and consistency

2X Tris-SDS Buffer, pH 8.8 (Gel Casting Buffer – For PAGE)

2X Tris-SDS Buffer is a concentrated, pH optimised solution of Tris-SDS intended for use as the casting buffer in SDS-PAGE of proteins.

Applications

• SDS-PAGE
• Western Blotting

Tris-SDS Buffer, pH: 8.8 (Gel Casting Buffer – For PAGE)

Ready-to-use buffer for SDS-PAGE

• Reduce preparation time and eliminate a source of error